Quantification Kits

Quantification Kits

ExoTEST™ Ready-to-Use Exosome Capture & Quatification Kits

ExoTEST is a patented double sandwich ELISA assay for quantitative and qualitative analysis of exosomes. In particular ExoTEST is a successful platform for exosomes quantification and characterization from small amount of human biological fluids or cell media and it may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions.

ExoTEST consists in ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from different biological samples, including cell culture supernatants and human biological fluids. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/tissue-specific exosomes. Lyophilized Exosome Standards, charcterized for protein content and particle number (NTA) allow the quantification of unknown sample by a standard calibration curve.

Applications: Exosome capture and quantification from human biofluids and cell culture media. Exosome comprehensive profiling. Pre-clinical research on non-invasive biomarkers for detection and monitoring of a number of pathological conditions (inflammation, cancer, neurodegeneration, etc.).

Advantages: Ready to use, No initial exosome purification required. User friendly and suitable for multiple marker analyses. Available in TEST format (limited to 3 ELISA strips, 24 wells).

Specifications: 

Description

ExoTEST™ is a sandwich enzyme-linked immunoassay for the in vitro quantitative measurement of overall exosome in human serum, plasma, urine and cell culture supernatant

Specificity

This assay has high sensitivity and excellent specificity for detection of exosomes. No significant cross-reactivity or interference were observed with any other soluble antigen or other-than exosome vesicles type.

Amount of starting material

Starting material : Plasma, Urine, Serum or Cell Culture Supernatant

 

Up to 100µL of biological sample can be loaded per well

Unfractionated plasma sample can be directly used for capture

Concentrated urine and cell culture supernatant samples are recommended prior capture according to our suggested protocol

Lower Detection Limit

The detection limit of an assay is lower than 0.35μg of overall exosome which is an equivalent of less than 50 pg of targeted exosomes protein

Exosome capture and quantification

Exosomes Binding

Sample incubation on immunoplate coated with exosome marker specific antibody (details in Chapter 1 regarding Exosome Immunocapture and Isolation Tools).

 

Assay Calibration

Calibration standard, lyophilized exosomes preparation 100µg vials from plasma or urine of healthy donors, COLO1, MM1 or BLCL cell line  (Description in Chapter 2 regarding Exosome Standards)

 

Primary Detection and Signal Reading 

Primary detection Antibody (depends on the kit)

Secondary HRP-conjugated antibody (kits with transparent and white plates)

Blocking reagent

Substrate solution (kits with transparent and white plates)

Stop solution (only in kit with transparent plate)

Shipment and storage

All the reagent are shipped at 4°C and storage conditions as recommended in the product insert

Expiration date

12 months