Hyaluronic Acid Oligosaccharides
Hyaluronic Acid (HA) is a glycosaminoglycan composed of an alternating sequence of β1,3 glucuronic acid (GlcA) and β1,4 N-acetylglucosamine (GlcNAc). In its native state HA is normally present in the extracellular matrix as a high molecular weight, high viscosity polymer essential for maintenance tissue architecture, elasticity and hydration. However it also has other key functions including the regulation of cell behaviour through specific interactions with cell surface receptors and extracellular proteins. HA binding to individual proteins commonly involves relatively short sequences in the HA polymer and there is considerable evidence that HA fragments generated in vivo have distinctive properties from the intact polymer.
Iduron offers a range of oligosaccharides produced by controlled endolyase scission of purified, low endotoxin Hyaluronic Acid (Streptococcal species). The oligosaccharides are separated by high resolution gel filtration and purity assessed on an analytical Superdex S75 HPLC column (see Data Sheets for profiles).
Size range of oligosaccharides dp2 to dp20. (dp is degree of monosaccharide polyimerisation - dp2 is a disaccharide, dp4 is a tetrasaccharide etc.)
General formula: ∆HexAβ1,3 - [GlcNAcβ1,4 GlcAβ1,3]n - GlcNAc
n = number of disaccharide units
∆HexA is the C4-C5 unsaturated hexuronic acid at the non-reducing end of the oligosaccharides produced by endolyase scission of the HA polymer. The C4-C5 double bond absorbs strongly at 232nm and can be used for monitoring the oligosaccharides in various separation systems.
Hyaluronic acid polymers also available covering size range from 93,000 to 1,100,000 daltons (93kDa to 1,100kDa).