Larger Quantities Available Upon Request
- Ultrapure quality
- Binding specificity for N-acetyl-D-galactosamine
- Specificity for blood group: A1 > A2 >> B
- Lyophilized powder
Glycine max lectin is isolated from soy bean (Soy bean agglutinin, SBA) and purified by affinity chromatography. The molecular weight of the lectin is 120 kDa, it consists of four identical subunits of 30 kDa each and it displays carbohydrate binding specifity for N-acetyl-D-galactosamine and galactopyranosyl residues of glycoproteins (1).
SBA has specificity for blood groups A1, A2 and B. The lectin interacts better with neuramidase-treated cells than with untreated cells. It has selective affinity for lymphocytes and human CD34+ hematopoietic stem cells. Immobilized conjugates of SBA are therefore important tools for removing T-cells in bone marrow transplantation.
Medicago’s Glycine max lectin is supplied as a white to cream coloured lyophilized powder from a buffer containing 50 mM NH4HCO3, no preservatives are added. The material is made from a single production batch and is homogenous. It is not derived from any material of animal origin. The lectin is available in vials containing 50 mg or 10 mg lyophilized powder and the product is to be used for laboratory work only.
- Studies of SBA-binding normal and tumour cells
- Blood group agglutination
- Glycoprotein studies
- Cell agglutination studies
Directions for use
The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol. The solution may be reconstituted in this buffer to desired working concentration. In absence of lactose the lectin will polymerize and storage at pH 8.6–8.8 causes precipitation.
Shipping and storage
The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.
Appearance: White to cream coloured lyophilized powder
Source: Soy bean
Molecular weight: 120 kDa
Sugar specificity: D-GalNAc
Activity: Less than 4 μg/ml will agglutinate fresh A1 cells. Older B cells can be stronger than A2 cells. Blood group specificity: A1 > A2 >> B
Microorganisms: < 100 CFU/g
Protein content: > 80 %, OD280nm (ε 1.28 = 1 mg/mL)
Identity: SDS-PAGE, one band corresponding to the four identical subunits
Shelf life: Three years when stored at -20°
(1) Liener I. E., Sharon N., Goldstein I. J., (1986) The Lectins – Properties, Functions and Applications in Biology and Medicine.
(2) X-ray crystallographic studies of unique cross-linked lattices between four isomeric biantennary oligosaccharides and soybean agglutinin. Olsen, L.R., Dessen, A., Gupta, D., Sabesan, S., Sacchettini, J.C., Brewer, C.F. (1997) Biochemistry 36: 15073–80.