Crotalaria juncea lectin

$392.00
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SKU:
05-0105-10mg
Weight:
8.00 LBS
Shipping:
Calculated at Checkout

Description

Larger Quantities Available Upon Request

Features 

  • Ultrapure quality
  • Non-specific blood group activity
  • Sugar specificity: Gal > GalNAc
  • Phytohaemagglutinin
  • Lyophilized powder

 

 

 

 

 

Product description

Crotalaria juncea lectin is isolated from Sunn hemp seeds and purified by affinity chromatography. The lectin has a molecular weight of 124 kDa and is composed of four identical polypeptide chains of 31 kDa. The lectin has non-specific blood group activity (1). It displays specificity toward ß-galactosides and interacts with serum glycoproteins, cytochrome b5 and virus surface glycoproteins such as BVDV, influenza and bovine diarrhea (1).

Medicago’s Crotalaria juncea is supplied as a white to yellowish lyophilized powder. The purity ofCrotalaria juncea lectin is determined by SDS- PAGE, which generates one single band at 31 kDa corresponding to the four identical polypeptide chains. The lectin is available in vials containing 50 mg or 10 mg powder. The product is to be used for laboratory work only.

 

Applications

  • Studies of virus surface glycoproteins
  • Purifying bovine diarrhea virus when immobilized an agarose gel

 

Directions for use

The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol. The solution may be reconstituted in this buffer to desired working concentration. In absence of lactose the lectin will polymerize and storage at pH 8.6–8.8 causes precipitation.

 

Shipping and storage

The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.

 

Specifications

Appearance: White to yellowish lyophilized powder                              

Source: Sunn hemp seeds

Molecular weight: 124 kDa

Sugar specificity: Gal > GalNAc

Activity: Blood group non-specific

Microorganisms: < 100 CFU/g

Protein content: > 90 %, OD280nm (ε 1mg/ml = 1.14), > 95%, essentially salt free

Identity: SDS-PAGE, one band at 31 kDa

Shelf life: > Three years when stored at -20°

 

References

(1) Liener I. E., Sharon N., Goldstein I. J., (1986) The Lectins – Properties, Functions and Applications in Biology and Medicine.