Bioengineered Heparan Sulfate
TEGA is the first company to produce bioengineered heparan sulfate. Heparan sulfate (HS) is a type of GAG found on the surface of all human cells and is a major component of the extracellular matrix. Hundreds of proteins bind to HS, which drives a wide variety of processes in human development and physiology across all organ systems. A major limitation to experimental research on HS in the lack of high quality material that is structurally and functionally characterized.
To date, commercial vendors have provided small quantities of HS for research, usually derived from kidney or as a byproduct of heparin production from porcine intestinal mucosa. Animal tissue derived HS suffers from batch to batch variation, carries a risk of contamination and fails to reproduce the variety of compositions found in nature.
TEGA recombinant Heparan Sulfate (rHS) is produced using engineered cell lines in a controlled process that results in a high quality product that is not susceptible to the issues of animal sources and potential contamination. TEGA confirms batch-to-batch consistency and quality by testing rHS composition and performance.
Cell line engineering enables production of natural variation in heparan sulfate characteristics including disaccharide composition, sulfation and protein binding. TEGA’s rHS library variants demonstrate different and varied assay, cellular and physiological responses.